Organization and dynamics of the Mu transpososome: recombination by communication between two active sites

摘要: Movement of transposable genetic elements requires the cleavage each end element genome and subsequent joining these cleaved ends to a new target DNA site. During Mu transposition, reactions are catalyzed by tetramer four identical transposase subunits bound paired ends. To elucidate organization active sites within this tetramer, subunit providing essential site DDE residues for reaction was determined. We demonstrate that recombination two is sites, where one promotes both end. This uses all three from binding proximal on other (catalysis in trans). In addition, we uncover evidence catalytic activity coupled such coordinated favored during recombination. On basis results, propose stage cooperative transition transposase–DNA complex. The utilization supplied trans provides an example how mobile can ensure concomitant distant sites.