A truncated form of the bacteriophage Mu B protein promotes conservative integration, but not replicative Transposition, of Mu DNA

摘要: Summary The phage-encoded proteins required for conservative integration of infecting bacteriophage Mu DNA were investigated. Our findings show that functional gp A , an essential component the phage transposition system, is integration. B protein, which greatly enhances replicative DNA, also required. Furthermore, a truncated form lacking 18 amino acids from carboxy terminus blocked in transposition, but not results point to more prominent role gpB than simply replication enhancer transposition. ability function integration, suggests key carboxy-terminal domain protein reaction. existence shortened uncouples should be invaluable future dissection