Reorganization of the Mu Transpososome Active Sites during a Cooperative Transition between DNA Cleavage and Joining

摘要: Transposition of mobile genetic elements proceeds through a series DNA phosphoryl transfer reactions, with multiple reaction steps catalyzed by the same set active site residues. Mu transposase repeatedly utilizes DDE residues to cleave and join single strand at each transposon end new, distant location (the target DNA). To better understand how is manipulated within transposase-DNA complex during recombination, impact immediately adjacent ends flanking DNA) on progress transposition was investigated. We show that, in absence MuB activator, 3 ′-flanking can slow one or more between cleavage joining. The presence this just slows joining step both sites. Further evidence suggests that not due change affinity transpososome for DNA. Finally, we demonstrate activates stimulating otherwise slowed strand. Based these results, propose must be removed from, shifted within, sites after step; movement coupled conformational properly positions simultaneously thereby permits