Autovaccination Confers Protection against Devriesea agamarum Associated Septicemia but Not Dermatitis in Bearded Dragons (Pogona vitticeps)

摘要: Devrieseasis caused by Devriesea agamarum is a highly prevalent disease in captive desert lizards, resulting severe dermatitis and some cases mass mortality. In this study, we assessed the contribution of autovaccination to devrieseasis control evaluating capacity 5 different formalin-inactivated D. vaccines induce humoral immune response bearded dragons (Pogona vitticeps). Each vaccine contained one following adjuvants: CpG, incomplete Freund's, Ribi, aluminium hydroxide, or curdlan. Lizards were administrated through subcutaneous injection booster vaccination was given 3 weeks after primo-vaccination. An indirect ELISA developed used monitor lizard serological responses. Localized adverse effects immunization observed all but Ribi adjuvanted group. Following homologous experimental challenge, Freund's as well confer protection against development associated septicemia not dermatitis. Subsequently, two-dimensional gelelectrophoresis followed immunoblotting spectrometry conducted with serum obtained from lizards that showed seroconversion immunisation vaccine. Fructose-bisphosphate aldolase aldo-keto reductase reacted latter lizards. Based on demonstrated partial use identification target antigens vaccinated dragons, study provides promising information towards strategy collections.