Evidence for mitogen-associated protein kinase activation and transduction of mitogenic signals by platelet-derived growth factor in human meningioma cells.

摘要: Object. Coexpression of platelet-derived growth factor (PDGF)—BB and activated PDGF-β receptor in meningioma cells indicates that this cytokine may act as an autocrine or paracrine stimulant growth. The intracellular events transducing signals from tyrosine kinases are unknown. In study the authors evaluated whether not mitogen-activated protein (MAPKs) expressed meningiomas, regulate their growth, transduce mitogenic PDGF-BB. Methods. Ten human tumors well cultured two normal leptomeninges 10 additional meningiomas were using Western blot analysis to determine presence MAPK phosphorylated (activated) MAPK. effects PD098059, a selective inhibitor phosphorylation/activation, on proliferation stimulated with 10% fetal bovine serum was also evaluated. Last, PDGF-BB stimulation associated act...