The Impact of Low-Level Iron Supplements on the Faecal Microbiota of Irritable Bowel Syndrome and Healthy Donors Using In Vitro Batch Cultures.

摘要: Ferrous iron supplementation has been reported to adversely alter the gut microbiota in infants. To date, impact of on adult is limited, particularly at low supplementary concentrations. The aim this research was explore low-level healthy and Irritable Bowel Syndrome (IBS) volunteers. Anaerobic, pH-controlled vitro batch cultures were inoculated with faeces from or IBS donors along (ferrous sulphate, nanoparticulate pea ferritin (50 μmol−1 iron)). explored by fluorescence situ hybridisation coupled flow cytometry. Furthermore, metabolite production assessed gas chromatography. volunteers had different starting microbial profiles controls. sources did not negatively population, results being similar iron, whilst ferrous sulphate led enhanced Bacteroides spp. data suggested no shift potentially negative proteolysis. indicate that doses three detrimental microbiota. This first time fermentation tested indicates dose unlikely be