Intraspinal and Intracortical Delivery of AAV Vectors for Intersectional Circuit Tracing in Non-transgenic Species.

摘要: The mapping of long-range axonal projections is a rapidly growing endeavor in the field neuroscience. Recent advances development adeno-associated viral vectors that can achieve efficient retrograde transport now enable characterization and manipulation specific neuronal subpopulations using Cre-dependent, intersectional approaches. Importantly, these approaches be applied to non-transgenic animals, making it possible carry out detailed anatomical studies across variety species including nonhuman primates. In this chapter, we demonstrate utility such strategies by describing methods for targeting constructs distinct subsets corticospinal motor neurons based on their spinal cord segments.