MoeH5: a natural glycorandomizer from the moenomycin biosynthetic pathway.
作者： Bohdan Ostash , Jennifer Campbell , Andriy Luzhetskyy , Suzanne Walker
DOI: 10.1111/MMI.12437
关键词: Biology 、 Biochemistry 、 Secondary metabolism 、 Streptomyces 、 Enzyme 、 Streptomyces ghanaensis 、 Glutamine amidotransferase 、 Biosynthesis 、 Genetics 、 Regulation of gene expression 、 Gene
摘要: The biosynthesis of the phosphoglycolipid antibiotic moenomycin A attracts attention researchers hoping to develop new moenomycin-based antibiotics against multidrug resistant Gram-positive infections. There is detailed understanding most steps this biosynthetic pathway in Streptomyces ghanaensis (ATCC14672), except for ultimate stage, where a single pentasaccharide intermediate converted into set unusually modified final products. Here we report that only one gene, moeH5, encoding homologue glutamine amidotransferase (GAT) enzyme superfamily, responsible observed diversity terminally decorated moenomycins. Genetic and biochemical evidence support idea MoeH5 novel member GAT whose homologues are involved synthesis various secondary metabolites as well K O antigens bacterial lipopolysaccharide. Our results provide insights mechanism its counterparts, give us tool diversification antibiotics.
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