An Integrated Approach to Proteome Analysis: Identification of Proteins Associated with Cardiac Hypertrophy

摘要: Abstract Hypertrophy of cardiac myocytes is a primary response the heart to overload, and an independent predictor failure death. Distinct cellular phenotypes are associated with hypertrophy resulting from different causes. These have been described by others at molecular level analysis gene transcription patterns. An alternative approach large-scale protein expression patterns (the proteome) two-dimensional polyacrylamide gel electrophoresis. Realization this goal requires ability rigorously analyze complex 2D images, efficiently digest individual isolated proteins (especially those expressed low levels), peptides high sensitivity for rapid database searches. We undertaken improve technology experimental approaches these challenges in order effectively study cell culture model hypertrophy. The lysates multiple samples or without phenylephrine-induced were analyzed spots which changed abundance statistical significance located. Eleven such identified usingimproved procedures in-gel digestion silver-stained high-sensitivity mass spectrometry. incorporation levels sodium dodecyl sulfate into buffer improved peptide recovery. combination matrix-assisted laser desorption spectrometry initial measurements capillary liquid chromatography–ion trap sequence determination yielded efficient identification. integration image routine identification present gels subpicomole represents general proteome studies relating genomic