Arginine methylation of the c‐Jun coactivator RACO‐1 is required for c‐Jun/AP‐1 activation

摘要: c-Jun, the major component of AP-1 transcription factor complex, has important functions in cellular proliferation and oncogenic transformation. The RING domain-containing protein RACO-1 as a c-Jun coactivator that molecularly links growth signalling to transactivation. Here we demonstrate is present nuclear dimer specifically interacts with dimeric RACO-1. Moreover, identified substrate arginine methyltransferase PRMT1, which methylates on two residues. Arginine methylation promotes conformational change stabilises by facilitating K63-linked ubiquitin chain formation, enables dimerisation interaction. Abrogation PRMT1 function impairs activity results decreased expression large percentage target genes. These required for efficient transcriptional activation c-Jun/AP-1 thus identify an regulator function.