Identification of a human liver cytochrome P-450 homologous to the major isosafrole-inducible cytochrome P-450 in the rat.

摘要: The rat 3-methylcholanthrene-inducible family of liver cytochromes P-450 contains two proteins (P-450c and P-450d) that are immunochemically related, possess 68% total sequence homology, induced by a number toxic or carcinogenic compounds. To determine whether equivalent isozymes hepatic cytochrome expressed in humans, as they several mammalian species, we performed immunoblot analyses on microsomes prepared from 14 human specimens found each one contained 52.5-kDa protein (termed HLd) reacted with antibodies specific for P-450d. In addition, specimen 54-kDa HLc) P-450c. HLd was purified through the use immunoaffinity chromatography to be 56% homologous P-450d 61% isozyme rabbit (P-450 LM4) their first 18 NH2-terminal amino acids. Finally, levels immunoreactive varied more than 10-fold among these patients but were unrelated patients' drug treatments, smoking habits, amount HLp, related glucocorticoid-inducible P-450. We conclude that, man, there is composed (HLc structurally observed other species.