A reliable semiautomated method for the determination of total thiamine in whole blood by the thiochrome method with high-performance liquid chromatography.
作者： Jaap Schrijver , Andries J Speek , Jan A Klosse , Herman J M Van Rijn , Wil H P Schreurs
DOI: 10.1177/000456328201900111
关键词: Chromatography 、 Hplc method 、 Chemistry 、 Vitamin 、 Thiamine 、 Enzymatic hydrolysis 、 Thiamine phosphate 、 High-performance liquid chromatography 、 Whole blood 、 Extraction (chemistry)
摘要: A sensitive and reliable method for the determination of total thiamine (vitamin B1) in whole blood has been developed which is suited routine analysis. After extraction, enzymatic hydrolysis phosphate esters, separated from interfering compounds by a fully automated high-performance liquid chromatographic (HPLC) system. Thiamine detected fluorometrically as thiochrome. By calculating concentration on-line with aid computer, it possible to complete one analysis within four hours. Routine determinations can be carried out series 120 samples 48 hours.The within-assay between-assay coefficients variation were 4·2 4·4%, respectively. The analytical recovery diphosphate added was 99·9 ± 11·7% (mean SD). HPLC described applied also plasma erythrocytes. In agreement wit...
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sci-hub.se 参考文章(11)
H. E. SAUBERLICH, Biochemical alterations in thiamine deficiency--their interpretation. The American Journal of Clinical Nutrition. ,vol. 20, pp. 528- 542 ,(1967) , 10.1093/AJCN/20.6.528
H. N. Haugen, The determination of thiamine and thiamine phosphates in blood and tissues by the thiochrome method. Scandinavian Journal of Clinical & Laboratory Investigation. ,vol. 13, pp. 50- 56 ,(1961) , 10.3109/00365516109137248
T. Van De Weerdhof, M.L. Wiersum, H. Reissenweber, Application of liquid chromatography in food analysis Journal of Chromatography A. ,vol. 83, pp. 455- 460 ,(1973) , 10.1016/S0021-9673(00)97062-6
Kazuhiko Ishii, Keisuke Sarai, Hiroshi Sanemori, Takashi Kawasaki, Analysis of thiamine and its phosphate esters by high-performamze liquid chromatography Analytical Biochemistry. ,vol. 97, pp. 191- 195 ,(1979) , 10.1016/0003-2697(79)90345-2
Hannu K. Penttinen, Olof Theander, Per Åman, O. Hänninen, Jean Vialle, T. Anthonsen, Differences in Thiochrome Fluorescence Produced by Thiamine and Its Mono-, Di-, and Triphosphate Esters. Acta Chemica Scandinavica. pp. 659- 663 ,(1976) , 10.3891/ACTA.CHEM.SCAND.30B-0659
Mieko Kimura, Tomio Fujita, Shigeki Nishida, Yoshinori Itokawa, Differential fluorimetric determination of picogram levels of thiamine, thiamine monophosphate, diphosphate and triphosphate using high-performance liquid chromatography Journal of Chromatography A. ,vol. 188, pp. 417- 419 ,(1980) , 10.1016/S0021-9673(00)81267-4
Jesse F. Gregory, Comparison of high-performance liquid chromatographic and Saccharomyces uvarum methods for the determination of vitamin B6 in fortified breakfast cereals. Journal of Agricultural and Food Chemistry. ,vol. 28, pp. 486- 489 ,(1980) , 10.1021/JF60229A029
Myron Brin, Erythrocyte as a biopsy tissue for functional evaluation of thiamine adequacy. JAMA. ,vol. 187, pp. 762- 766 ,(1964) , 10.1001/JAMA.1964.03060230090022
Herman Baker, Allan D. Thomson, Oscar Frank, Carroll M. Leevy, Absorption and passage of fat- and water-soluble thiamin derivatives into erythrocytes and cerebrospinal fluid of man The American Journal of Clinical Nutrition. ,vol. 27, pp. 676- 680 ,(1974) , 10.1093/AJCN/27.7.676
Gilbert A. Leveille, Modified thiochrome procedure for the determination of urinary thiamin The American Journal of Clinical Nutrition. ,vol. 25, pp. 273- 274 ,(1972) , 10.1093/AJCN/25.3.273