Oral treatment with the herbal formula B307 alleviates cardiac toxicity in doxorubicin-treated mice via suppressing oxidative stress, inflammation, and apoptosis.

摘要: Objective This study aimed to investigate whether the herbal formula B307 could alleviate doxorubicin (DOX)-induced acute cardiotoxicity. If so, we further unraveled possible molecular mechanisms of cardiac protection under treatment with B307. Methods Before animal experiment, examined relative viabilities Huh7 cancer cells To test oral cardiotoxicity, equal volumes (50 mg/kg) or saline (sham treatment) were administered 20-week-old male mice once daily for 14 consecutive days. Then, DOX (10 mg/kg; ip) was and sham treatments at 22-23 weeks age. Cardiac functions in these assessed via echocardiography 23-24 expressions oxidative stress, inflammation, apoptosis-related proteins heart tissue by immunohistochemistry Western blotting 24-25 Apart from this, mortality rate body weight measured during experiment. Results In vitro, had shown no obvious change doses 10-160 ng/mL. Furthermore, significantly reduced but showed significant only plus treatment. vivo, rate, weight, function DOX-treated obviously improved endothelial nitric oxide synthase, superoxide dismutase 2, B-cell lymphoma 2 enhanced, tumor necrosis factor alpha, NFKB1 (p50 its precursor, p105), neurotrophin-3, Bcl-2-associated X protein, calpain, caspase 12, 9, 3 suppressed Conclusion Our results revealed that may provide cardioprotection suppressing apoptosis tissue. We believe be developed as a potential alternative patients