NADPH-diaphorase in the developing rat: lower brainstem and cervical spinal cord, with special reference to the trigemino-solitary complex.

摘要: A previous study indicated that in adult rat, a distinctive neuronal group the dorsomedial division of subnucleus oralis spinal trigeminal nucleus (SpVo) and rostrolateral part solitary tract (Sn) is stained for nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d), suggested labeled structures are involved with sensorimotor reflexive functions. This aimed to characterize developmental expression NADPH-d SpVo Sn, including other areas lower brainstem cervical cord, by means enzyme histochemical staining technique, from prenatal through postnatal period. On embryonic day 12 (E12), no neurons brain were NADPH-d, whereas blood vessels stained. Labeling was consistently present throughout pre- periods but decreased development. E15, appeared not nuclei. The both nuclei increased numbers drastically E17. Postnatally, they tended increase gradually decrease slightly SpVo. cell size reached plateau at E17 SpVo, 4 (P4) Sn. In on E17, labeling lateral paragigantocellular reticular, intermediate medullary pedunculopontine tegmental, vestibular nuclei, laminae V, VI, X cord. E20 P0, dorsal column, laterodorsal raphe obscurus, parvocellular ventral gigantocellular parahypoglossal IX P4 parabrachial median medial caudolateral magnocellular zone caudalis (SpVc), III/IV P10, paratrigeminal superficial SpVc, I/II No newly any after P14. very early appearance which precedes elsewhere brainstem, suggests nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) system has an important role primitive orofacial Furthermore, pattern Sn NO/cGMP organized distinct manner different