Immunofluorescence test with antigen-loaded erythrocytes: detection of influenza virus specific IgG, IgA, and IgM antibodies.

作者: P. C. Döller , G. Döller , H. -J. Gerth

DOI: 10.1007/BF02124945

关键词: Complement fixation testVirusRed blood cellImmunofluorescenceAntibodySerologyAntigenVirologyOrthomyxoviridaeBiology

摘要: An immunofluorescence test (IFT) for the detection of influenza virus antibodies was established to supplement standard serological diagnostical complement fixation (CFT). Current strains (A/Philippines/2/ 82, A/Brazil/11/78, B/Singapore/222/79) were loaded on formalinized chicken erythrocytes. In contrast CFT, we can distinguish specific immunoglobulin classes against virus. Unlike IFT is subtype-specific. A recent infection be distinguished from a past by differentiation classes. Anticomplementary factors and hemolytic sera do not influence result IFT. does require cell cultures easy read.

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