High performance reversed phase chromatography of cholesterol and cholesteryl esters of human plasma lipoproteins
作者： Edward G. Perkins , David J. Hendren , John E. Bauer , Ali H. El-Hamdy
DOI: 10.1007/BF02534908
关键词: Methanol 、 Lipoprotein 、 Cholesteryl ester 、 Double bond 、 Cholesterylester transfer protein 、 Chromatography 、 Reversed-phase chromatography 、 Chemistry 、 Elution 、 Cholesterol 、 Organic chemistry 、 Cell biology 、 Biochemistry
摘要: Cholesterol and cholesteryl esters were separated according to their carbon number of double bonds by high performance reversed-phase chromatography (HPRC) using acetonitrile/chloroform/methanol (1∶1∶1, v/v) as a mobile phase. It was found that within the same equivalent (ECN) category, cholesterol with highest eluted ahead those lower bonds, thecis isomers eluting theirtrans partners. Thus, oleate (C27-18∶1c) elutes palmitate (C27-16∶0) elaidate (C27-18∶1t). Human lipoprotein, well rat liver esters, this technique.
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