Improved microtitre plate production of single chain Fv fragments in Escherichia coli.
作者： Michael Hust , Miriam Steinwand , Laila Al-Halabi , Saskia Helmsing , Thomas Schirrmann
DOI: 10.1016/J.NBT.2009.03.004
关键词: Computational biology 、 clone (Java method) 、 Escherichia coli 、 Antibody 、 Phage display 、 Monoclonal antibody 、 Peptide library 、 Molecular biology 、 In vitro 、 Functional genomics 、 Biology
摘要: The new era of functional genomics demands several antibodies as specific detection reagents for proteins, their complexes and post-translational modifications. Only in vitro antibody selection technologies are able to provide the required throughput generate these large numbers. Phage display is most widely used technology antibodies. major bottleneck a phage pipeline production monoclonal fragments screening further analysis. In this study, we describe development improved protocols single chain Fv (scFv) 96-well microtitre plates (MTPs) Escherichia coli. Four scFvs were expressed using expression vector pOPE101-XP analyse influence set different parameters on production. Further, six pHAL14 investigate effect those that from pOPE101-XP. Yield MTPs was influenced by variety conditions also strongly dependent individual scFv clone. Although it not possible deduce optimal applicable all tested scFvs, combined protocol developed which over standard methods.
elsevier.com
sci-hub.se 参考文章(39)
Michael Hust, André Frenzel, Thomas Schirrmann, Stefan Dübel, Selection of Recombinant Antibodies From Antibody Gene Libraries Methods of Molecular Biology. ,vol. 1101, pp. 305- 320 ,(2014) , 10.1007/978-1-62703-721-1_14
Stefan Dübel, Janice M Reichert, Handbook of therapeutic antibodies Wiley Blackwell,. pp. 1- 4 ,(2007) , 10.1002/9783527619740
Michael Hust, Holger Thie, Thomas Schirrmann, Stefan Dbel, Antibody Phage Display Therapeutic Monoclonal Antibodies. pp. 189- 211 ,(2009) , 10.1002/9780470485408.CH8
Edwin Boel, Sjors Verlaan, Miriam J.J.G Poppelier, Nomdo A.C Westerdaal, Jos A.G Van Strijp, Ton Logtenberg, Functional human monoclonal antibodies of all isotypes constructed from phage display library-derived single-chain Fv antibody fragments. Journal of Immunological Methods. ,vol. 239, pp. 153- 166 ,(2000) , 10.1016/S0022-1759(00)00170-8
Michael Hust, Stefan Dübel, Mating antibody phage display with proteomics Trends in Biotechnology. ,vol. 22, pp. 8- 14 ,(2004) , 10.1016/J.TIBTECH.2003.10.011
Michael Hust, Thomas Jostock, Christian Menzel, Bernd Voedisch, Anja Mohr, Mariam Brenneis, Martina I Kirsch, Doris Meier, Stefan Dübel, Single chain Fab (scFab) fragment BMC Biotechnology. ,vol. 7, pp. 14- 14 ,(2007) , 10.1186/1472-6750-7-14
David R. Buckler, Albert Park, Malini Viswanathan, Rene M. Hoet, Robert C. Ladner, Screening isolates from antibody phage-display libraries. Drug Discovery Today. ,vol. 13, pp. 318- 324 ,(2008) , 10.1016/J.DRUDIS.2007.10.012
M. Kirsch, M. Zaman, D. Meier, S. Dübel, M. Hust, Parameters affecting the display of antibodies on phage Journal of Immunological Methods. ,vol. 301, pp. 173- 185 ,(2005) , 10.1016/J.JIM.2005.04.017
Steffen Goletz, Peter Astrup Christensen, Peter Kristensen, Dietmar Blohm, Ian Tomlinson, Greg Winter, Uwe Karsten, Selection of large diversities of antiidiotypic antibody fragments by phage display. Journal of Molecular Biology. ,vol. 315, pp. 1087- 1097 ,(2002) , 10.1006/JMBI.2001.5314
A. Rolfs, W. R. Montor, S. S. Yoon, Y. Hu, B. Bhullar, F. Kelley, S. McCarron, D. A. Jepson, B. Shen, E. Taycher, S. E. Mohr, D. Zuo, J. Williamson, J. Mekalanos, J. LaBaer, Production and sequence validation of a complete full length ORF collection for the pathogenic bacterium Vibrio cholerae Proceedings of the National Academy of Sciences of the United States of America. ,vol. 105, pp. 4364- 4369 ,(2008) , 10.1073/PNAS.0712049105