Determination of the amino acid sequence of troponon C from rabbit skeletal muscle.
作者： J H Collins , M L Greaser , J D Potter , M J Horn
DOI: 10.1016/S0021-9258(17)39964-7
关键词: Histidine 、 Cysteine 、 Biochemistry 、 Cyanogen bromide 、 Tyrosine 、 Proline 、 Troponin C 、 Peptide sequence 、 Skeletal muscle 、 Chemistry
摘要: The complete amino acid sequence of rabbit skeletal muscle troponin C (TnC) was determined from studies on overlapping peptides isolated cyanogen bromide and tryptic digests. TnC found to be a single polypeptide chain 159 residues, including 2 residues tyrosine 1 each cysteine, histidine, proline. end is acetylated, the calculated net charge at pH 7.0 -29, molecular weight 17,965. There no evidence for heterogeneity in sequence. previously proposed four apparent Ca2+ binding sites are located 27 38, 63 74, 103 114, 139 150.
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