Detoxification enzymes of freshwater crayfish Procambarus clarkii fed a diet enriched in selenium: Preliminary results

作者: Antonia Concetta Elia , Ambrosius Josef Martin Dörr , Marino Prearo , Maria Illuminata Taticchi , Maria Cesarina Abete

DOI: 10.1080/10236240701556976

关键词: Glutathione reductaseBiologyProcambarus clarkiiSeleniumBiochemistryFood scienceCatalaseEnzymeGlutathione peroxidaseCrayfishHepatopancreas

摘要: This study aimed at investigating the extent of accumulation selenium in hepatopancreas crayfish Procambarus clarkii and its effects on enzymatic activities catalase, glutathione peroxidase, reductase. Crayfish were fed for 15 days either a diet enriched Se, ED (1.21 mg kg−1) or standard diet, SD (0.30 mg kg−1). Results showed that Se is higher sample subject to rather than SD. A sexual difference was found response analyzed enzymes only. Catalase activity peroxidase decreased females males, respectively, whereas reductase significant reductions both sexes. These preliminary data might provide information about prooxidant P. when diet.

参考文章(12)
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Madiha H. Helmy, Saad S. Ismail, Hassan Fayed, Emad A. El-Bassiouni, Effect of selenium supplementation on the activities of glutathione metabolizing enzymes in human hepatoma Hep G2 cell line. Toxicology. ,vol. 144, pp. 57- 61 ,(2000) , 10.1016/S0300-483X(99)00190-0
Azza I. Othman, Mohammed A. El Missiry, Role of selenium against lead toxicity in male rats. Journal of Biochemical and Molecular Toxicology. ,vol. 12, pp. 345- 349 ,(1998) , 10.1002/(SICI)1099-0461(1998)12:6<345::AID-JBT4>3.0.CO;2-V
Steven J. Hamilton, Review of selenium toxicity in the aquatic food chain. Science of The Total Environment. ,vol. 326, pp. 1- 31 ,(2004) , 10.1016/J.SCITOTENV.2004.01.019
A. C. ELIA, A. J. M. DÖRR, C. MASTRANGELO, M. PREARO, M. C. ABETE, GLUTATHIONE AND ANTIOXIDANT ENZYMES IN THE HEPATOPANCREAS OF CRAYFISH PROCAMBARUS CLARKII (GIRARD, 1852) OF LAKE TRASIMENO (ITALY) Bulletin Francais De La Peche Et De La Pisciculture. pp. 1351- 1361 ,(2006) , 10.1051/KMAE:2006040
Y. Seko, Y. Saito, J. Kitahara, N. Imura, Active Oxygen Generation by the Reaction of Selenite with Reduced Glutathione in Vitro Springer, Berlin, Heidelberg. pp. 70- 73 ,(1989) , 10.1007/978-3-642-74421-1_14
J. G. Bell, C. B. Cowey, J. W. Adron, Aileen M. Shanks, Some effects of vitamin E and selenium deprivation on tissue enzyme levels and indices of tissue peroxidation in rainbow trout (Salmo gairdneri). British Journal of Nutrition. ,vol. 53, pp. 149- 157 ,(1985) , 10.1079/BJN19850019