Population Biology of Intestinal Enterococcus Isolates from Hospitalized and Nonhospitalized Individuals in Different Age Groups

作者: Ana P Tedim , Patricia Ruiz-Garbajosa , Jukka Corander , Concepción M Rodríguez , Rafael Cantón

DOI: 10.1128/AEM.03661-14

关键词: PopulationGenotypeEnterococcus faeciumFecesMicrobiologyAntibiotic resistanceColonizationEnterococcusBiologyEnterococcus faecalis

摘要: The diversity of enterococcal populations from fecal samples hospitalized (n = 133) and nonhospitalized individuals 173) different age groups (group I, ages 0 to 19 years; group II, 20 59 III, ≥60 years) was analyzed. Enterococci were recovered at similar rates persons (77.44% 79.77%) all (75.0% 82.61%). Enterococcus faecalis faecium predominant, although seven other species identified. E. (including ampicillin-resistant faecium) colonization in independent. For inpatients, independent, but (particularly the colonization) significantly increased with age. population structure determined by superimposing goeBURST Bayesian analysis (BAPS). Most sequence types (STs; 150 isolates belonging 75 STs) linked BAPS 1 (22.0%), 2 (31.3%), 3 (36.7%). A positive association between hospital subgroups 2.1a 3.3a (which included major human lineages) community-based 1.2 3.3b found. (130 58 grouped into groups, (36.9%), (40.0%), (23.1%), each one comprising widespread lineages. No associations or hospitalization established. dynamics microbiota healthy humans are largely unexplored, available knowledge being fragmented contradictory. study offers a novel comprehensive landscapes suggests that patients differ, predominance certain clonal lineages, often elderly individuals, occurring setting.

参考文章(82)
Francois Lebreton, Michael S. Gilmore, Rob J. L. Willems, Enterococcus Diversity, Origins in Nature, and Gut Colonization Massachusetts Eye and Ear Infirmary. ,(2014)
Gerald W. Tannock, Greg Cook, Enterococci as Members of the Intestinal Microflora of Humans American Society of Microbiology. pp. 101- 132 ,(2002) , 10.1128/9781555817923.CH3
CJ Stewart, ECL Marrs, S Magorrian, A Nelson, C Lanyon, JD Perry, ND Embleton, SP Cummings, JE Berrington, The preterm gut microbiota: changes associated with necrotizing enterocolitis and infection Acta Paediatrica. ,vol. 101, pp. 1121- 1127 ,(2012) , 10.1111/J.1651-2227.2012.02801.X
W. van Schaik, R.J.L. Willems, Genome‐based insights into the evolution of enterococci Clinical Microbiology and Infection. ,vol. 16, pp. 527- 532 ,(2010) , 10.1111/J.1469-0691.2010.03201.X
Joseph M. Hilbe, James W. Hardin, Generalized Estimating Equations ,(2002)
Charisse Petersen, June L. Round, Defining dysbiosis and its influence on host immunity and disease. Cellular Microbiology. ,vol. 16, pp. 1024- 1033 ,(2014) , 10.1111/CMI.12308
T M Coque, B E Murray, Identification of Enterococcus faecalis strains by DNA hybridization and pulsed-field gel electrophoresis. Journal of Clinical Microbiology. ,vol. 33, pp. 3368- 3369 ,(1995) , 10.1128/JCM.33.12.3368-3369.1995
P Ruiz-Garbajosa, R Cantón, V Pintado, TM Coque, R Willems, F Baquero, R Del Campo, None, Genetic and phenotypic differences among Enterococcus faecalis clones from intestinal colonisation and invasive disease. Clinical Microbiology and Infection. ,vol. 12, pp. 1193- 1198 ,(2006) , 10.1111/J.1469-0691.2006.01533.X
M. J. Zervos, S. Donabedian, L. B. Jensen, M. V. Francia, F. Baquero, L. Peixe, A. R. Freitas, T. M. Coque, C. Novais, A. M. Hammerum, C. H. Lester, Human and Swine Hosts Share Vancomycin-Resistant Enterococcus faecium CC17 and CC5 and Enterococcus faecalis CC2 Clonal Clusters Harboring Tn1546 on Indistinguishable Plasmids Journal of Clinical Microbiology. ,vol. 49, pp. 925- 931 ,(2011) , 10.1128/JCM.01750-10