Studies on non-H-2-linked lymphocyte-activating determinants. IV. Ontogeny of the Mls product on murine B cells.

摘要: Abstract The minor lymphocyte stimulating (Mls) locus codes for activating determinants (LADs) on murine B lymphocytes, but not T lymphocytes. This observation was strengthened by a series of techniques which allow deletion and addition cells. These included the use cytotoxic antisera such as anti-Thy 1.2, anti-MTLA, anti-MBLA, complement, goat anti-μ antisera, finally fluorescence activated cell sorter (FACS). studies in this report document organ distribution ontogenetic appearance surface LADs lymphocytes from DBA/2N (H-2d, Mlsa) CBA/J (H-2k, Mlsd) mice. Adult-like ability to stimulate H-2 identical BALB/c Mlsb) C3H/He Mlsc) responder cells appeared at about 4–5 weeks age. Inability neonatal induce an Mls-defined MLC found be due low frequency or presence suppressor cells, absence Mls-coded their surface. data support concept that are present adult specific markers B-cell differentiation, is preceded membrane IgM δ homologue human IgD, Ia, receptor third component complement.