Multiple thioredoxin-mediated routes to detoxify hydroperoxides in Mycobacterium tuberculosis.
作者： Timo Jaeger , Heike Budde , Leopold Flohé , Ulrich Menge , Mahavir Singh
DOI: 10.1016/J.ABB.2003.11.021
关键词: Mycobacterium tuberculosis 、 Peroxynitrite 、 Biochemistry 、 Isoniazid 、 Biology 、 Mycothiol 、 Antioxidant 、 Thioredoxin reductase 、 Thioredoxin 、 Microbiology 、 Peroxiredoxin
摘要: Drug resistance and virulence of Mycobacterium tuberculosis are in part related to the pathogen’s antioxidant defense systems. KatG− strains resistant first line tuberculostatic isoniazid but need compensate their catalase deficiency by alternative peroxidase systems stay virulent. So far, only NADH-driven AhpD-mediated hydroperoxide reduction AhpC has been implicated as such virulence-determining mechanism. We here report on two novel pathways which underscore importance thioredoxin system for M. tuberculosis: (i) NADPH-driven that is mediated reductase C (ii) atypical peroxiredoxin TPx equally depends can use both, B C. Kinetic analyses with different hydroperoxides including peroxynitrite qualify redox cascade comprising reductase, C, most efficient protect against oxidative nitrosative stress situ.
elsevier.com
sci-hub.se 参考文章(50)
María Noel Alvarez, Madia Trujillo, Rafael Radi, Peroxynitrite formation from biochemical and cellular fluxes of nitric oxide and superoxide. Methods in Enzymology. ,vol. 359, pp. 353- 366 ,(2002) , 10.1016/S0076-6879(02)59198-9
R Radi, J S Beckman, K M Bush, B A Freeman, Peroxynitrite oxidation of sulfhydryls. The cytotoxic potential of superoxide and nitric oxide. Journal of Biological Chemistry. ,vol. 266, pp. 4244- 4250 ,(1991) , 10.1016/S0021-9258(20)64313-7
Dolph L. Hatfield, Selenium: Its Molecular Biology And Role In Human Health ,(2012)
Thomas C. Zahrt, Vojo Deretic, Reactive Nitrogen and Oxygen Intermediates and Bacterial Defenses: Unusual Adaptations inMycobacterium tuberculosis Antioxidants & Redox Signaling. ,vol. 4, pp. 141- 159 ,(2002) , 10.1089/152308602753625924
Joseph S. Beckman, Jun Chen, Harry Ischiropoulos, John P. Crow, [23] Oxidative chemistry of peroxynitrite Methods in Enzymology. ,vol. 233, pp. 229- 240 ,(1994) , 10.1016/S0076-6879(94)33026-3
G Storz, F S Jacobson, L A Tartaglia, R W Morgan, L A Silveira, B N Ames, An alkyl hydroperoxide reductase induced by oxidative stress in Salmonella typhimurium and Escherichia coli: genetic characterization and cloning of ahp. Journal of Bacteriology. ,vol. 171, pp. 2049- 2055 ,(1989) , 10.1128/JB.171.4.2049-2055.1989
Ruslana Bryk, Patrick Griffin, Carl Nathan, Peroxynitrite reductase activity of bacterial peroxiredoxins Nature. ,vol. 407, pp. 211- 215 ,(2000) , 10.1038/35025109
E. Pagán-Ramos, J. Song, M. McFalone, M. H. Mudd, V. Deretic, OXIDATIVE STRESS RESPONSE AND CHARACTERIZATION OF THE OXYR-AHPC AND FURA-KATG LOCI IN MYCOBACTERIUM MARINUM Journal of Bacteriology. ,vol. 180, pp. 4856- 4864 ,(1998) , 10.1128/JB.180.18.4856-4864.1998
G L Newton, K Arnold, M S Price, C Sherrill, S B Delcardayre, Y Aharonowitz, G Cohen, J Davies, R C Fahey, C Davis, Distribution of thiols in microorganisms: mycothiol is a major thiol in most actinomycetes. Journal of Bacteriology. ,vol. 178, pp. 1990- 1995 ,(1996) , 10.1128/JB.178.7.1990-1995.1996
S. S. Master, B. Springer, P. Sander, E. C. Boettger, V. Deretic, G. S. Timmins, Oxidative stress response genes in Mycobacterium tuberculosis: role of ahpC in resistance to peroxynitrite and stage-specific survival in macrophages. Microbiology. ,vol. 148, pp. 3139- 3144 ,(2002) , 10.1099/00221287-148-10-3139