Analysis of genetic relationships between 10 cattle breeds with 17 microsatellites

摘要: To guide genetic conservation programmes with objective criteria, general variability has to be taken into account. This study was conducted determine the variation between 10 cattle breeds by using 17 microsatellite loci and 13 biochemical markers (11 blood groups, transferrin β-casein loci). Microsatellite were amplified in 31–50 unrelated individuals from breeds: Charolais, Limousin, Breton Black Pied, Parthenais, Montbeliard, Vosgien, Maine-Anjou, Normande, Jersey Holstein. Neighbor-joining trees calculated distance estimates. The robustness of tree topology obtained bootstrap resampling loci. A total 210 alleles microsatellites detected this average heterozygosities ranged 0·53 breed 0·66 Parthenais breed. In general, low values obtained: microsatellites, highest concerned Holstein/Maine-Anjou grouping an occurrence 74%; markers, node had 79% Charolais/Limousin appeared when polymorphism analysed together, 90% that 42%. These results suggest 30 a number currently considered as sufficient distinguish closely related is, fact, probably insufficient.