Novel MicroRNA signatures in HPV-mediated cervical carcinogenesis in Indian women
作者： Shweta Sharma , Showket Hussain , Kartik Soni , Pallavi Singhal , Richa Tripathi
DOI: 10.1007/S13277-015-4248-7
关键词: Cancer 、 microRNA 、 Medicine 、 Real-time polymerase chain reaction 、 Cervical intraepithelial neoplasia 、 Pathology 、 Oncology 、 HPV infection 、 Internal medicine 、 Cervical cancer 、 Genotype 、 Carcinogenesis
摘要: This study aimed to investigate the role of miRNAs in HPV-mediated cervical pre-cancer and cancer cases Indian population. We analysed HPV infection its genotypes uterine (n = 80), (n = 200) normal samples (n = 150) by consensus sequence PCR followed type specific PCRs. Also, microRNA profiling was done a subset (n = 20), (n = 50) (n = 30) real-time quantitative (qRT-PCR). The prevalence found be 81 % (65/80) 94 % (188/200) cases, with most predominant high-risk type-16 (HR-HPV-16) 83 % 91 % pre- respectively. Whereas controls, very low (5 %). miRNA revealed that pre-cancer, 100 were significantly (p < 0.001) differentially expressed 70 upregulated 30 downregulated. In 383 (p < 0.001), which 350 33 also observed 182 HPV-16/18-positive (SiHa/HeLa) cell lines compared HPV-negative (C33A) line. addition, we identified novel microRNAs such as miR-892b, miR-500, miR-888, miR-505 miR-711 precancerous lesions Taken together, demonstrates crucial cancer, may serve potential early diagnostic markers for carcinogenesis.
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