MicroRNA expressions in HPV-induced cervical dysplasia and cancer.

摘要: Background/Aim: The role of oncogenic or high- risk human papillomavirus (HPV) in cervical carcinogenesis is inevitable, yet not fully understood. Detailed analysis microRNA (miRNA) alterations occurring during high-risk HPV transformation will increase our current understanding over carcinogenesis. two main aims the study were: (i) finding association between infection characteristics and socio-demographic variables, (ii) an predictors clinical outcome. Materials Methods: expression levels different microRNAs (miR-21, miR- 27a, miR-34a, miR-155, miR-196a, miR-203) were determined formalin-fixed paraffin-embedded (FFPE) HPV-positive dysplastic tumorous tissue samples using quantitative real-time PCR (qPCR). Sociodemographic life-style factors also analyzed. Results: miR-27a was significantly higher intraepithelial neoplasia (CIN)2-3 compared to CIN1 (p=0.023) squamous cell carcinoma (SCC) CIN2-3 (p=0.033). Moreover, lower miR-34a detected than (p=0.041) SCC (p=0.021). Furthermore, we found significant differences subjects with multiple (p=0.015) miR-203 (p=0.025) miR-21 (p=0.002), mir-27a (p=0.001) SCC/CIN2-3. Expression miR-27a, showing up-regulation (p=0.028) (down- regulated), correlated 16 positivity (CIN2-3/CIN1: p=0.027 SCC/CIN2-3: p=0.036). MiR-34a altered connection smoking status presence 16. Conclusion: demand for additional, alternative molecular biomarkers prognostic potential strong. Evaluation miRNA might be helpful distinguish lesions able help prediction Based on latest worldwide cancer statistics, third most commonly diagnosed females