MAP Kinase and Protein Kinase A–Dependent Mobilization of Triacylglycerol and Glycogen during Appressorium Turgor Generation by Magnaporthe grisea

作者: Eckhard Thines , Roland W. S. Weber , Nicholas J. Talbot

DOI: 10.1105/TPC.12.9.1703

关键词: GlycogenAppressoriumLipid dropletMagnaporthe griseaBiologyVacuoleTurgor pressureBiochemistryProtein kinase AMutant

摘要: Magnaporthe grisea produces an infection structure called an appressorium, which is used to breach the plant cuticle by mechanical force. Appressoria generate hydrostatic turgor by accumulating molar concentrations of glycerol. To investigate the genetic control and biochemical mechanism for turgor generation, we assayed glycerol biosynthetic enzymes during appressorium development, and the movement of storage reserves was monitored in developmental mutants. Enzymatic activities for glycerol generation from carbohydrate …

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