作者: Eckhard Thines , Roland W. S. Weber , Nicholas J. Talbot
关键词: Glycogen 、 Appressorium 、 Lipid droplet 、 Magnaporthe grisea 、 Biology 、 Vacuole 、 Turgor pressure 、 Biochemistry 、 Protein kinase A 、 Mutant
摘要: Magnaporthe grisea produces an infection structure called an appressorium, which is used to breach the plant cuticle by mechanical force. Appressoria generate hydrostatic turgor by accumulating molar concentrations of glycerol. To investigate the genetic control and biochemical mechanism for turgor generation, we assayed glycerol biosynthetic enzymes during appressorium development, and the movement of storage reserves was monitored in developmental mutants. Enzymatic activities for glycerol generation from carbohydrate …