A Novel Subnucleocapsid Nanoplatform for Mucosal Vaccination against Influenza Virus That Targets the Ectodomain of Matrix Protein 2

摘要: In this study, subnucleocapsid nanorings formed by the recombinant nucleoprotein (N) of respiratory syncytial virus were evaluated as a platform to anchor heterologous antigens. The ectodomain influenza A matrix protein 2 (M2e) is highly conserved and elicits protective antibodies when it linked an immunogenic carrier, making promising target develop universal vaccines. context, one or three M2e copies genetically C terminus N produce N-M2e N-3M2e chimeric nanorings. Mice immunized intranasally with 3M2e control peptides. N-3M2e-vaccinated mice showed strongest mucosal systemic antibody responses. These presented reduced viral load minor weight loss, all survived upon challenge A/PR8/34 (H1N1) (PR8). We compared intranasal route subcutaneous immunization. Only induced strong local IgA response led protection challenge. Finally, we demonstrated that induction anti-M2e not impaired preexisting anti-N immunity. Overall, these results show nanoring potent carrier for delivery vaccinal