Dynamics of Cell Ensembles on Adhesive Micropatterns: Bridging the Gap between Single Cell Spreading and Collective Cell Migration

摘要: The collective dynamics of multicellular systems arise from the interplay a few fundamental elements: growth, division and apoptosis single cells; their mechanical adhesive interactions with neighboring cells extracellular matrix; tendency polarized to move. Micropatterned substrates are increasingly used dissect relative roles these processes control resulting dynamics. Here we show that unifying computational framework based on cellular Potts model can describe experimentally observed cell over all relevant length scales. For cells, correctly predicts statistical distribution orientation axis as well final organisation two daughters large range micropatterns, including those situations in which stable configuration is not achieved rotation ensues. Large ensembles migrating heterogeneous environments form non-adhesive regions inward-curved arcs like epithelial bridge formation. Collective migration leads swirl formation variations area experimentally. In each case, also use our predict patterns have been studied before.