Rapid effects of a thiocarbamate herbicide and its dichloroacetamide protectant on macromolecular syntheses and glutathione levels in maize cell cultures

摘要: Abstract The rapid effects of the thiocarbamate herbicide S -ethyl dipropyl (EPTC) and protectant N,N -diallyl-2,2-dichloroacetamide (DDCA) on macromolecular syntheses glutathione (GSH) levels in maize cell cultures were studied to determine whether stimulation GSH could be primary mechanism action DDCA. EPTC (0.5 1 m M ) reduced incorporation radioactive precursors within hr after treatment, affected [ 3 H]acetate into lipids more than H]adenosine acid-precipitable nucleic acids, or 14 C]protein hydrolysate protein. C]EPTC was rapidly biotransformed 8 by suspensions. Measureable decreases following treatment with occurred 15 hr. DDCA stimulated 4 but did not affect protein acids. increases began 12 Treatment combination inhibited less given alone. Increases observed pretreatments precursors, no activity detected, contrast treatments It is suggested that has an initial effect lipid metabolism followed a slower involving cellular GSH.