Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport.
作者： M. R. Block , B. S. Glick , C. A. Wilcox , F. T. Wieland , J. E. Rothman
关键词: Biochemistry 、 Transport protein 、 Vesicular transport protein 、 Protein-fragment complementation assay 、 N-ethylmaleimide sensitive fusion protein 、 Chemistry 、 Vesicle 、 Tetramer 、 Soluble NSF attachment protein 、 Golgi apparatus
摘要: N-Ethylmaleimide (NEM) inhibits protein transport between successive compartments of the Golgi stack in a cell-free system. After inactivation membranes by NEM, can be rescued adding back an appropriately prepared cytosol fraction. This complementation assay has allowed us to purify NEM-sensitive factor, which we term NSF. The factor is tetramer 76-kDa subunits, and appears act catalytically, one leading metabolism numerous vesicles.
core.ac.uk
harvard.edu
sci-hub.se 参考文章(19)
W E Balch, J E Rothman, B W Wattenberg, A novel prefusion complex formed during protein transport between Golgi cisternae in a cell-free system. Journal of Biological Chemistry. ,vol. 261, pp. 2202- 2207 ,(1986) , 10.1016/S0021-9258(17)35918-5
Transport of the vesicular stomatitis glycoprotein to trans Golgi membranes in a cell-free system. Journal of Biological Chemistry. ,vol. 262, pp. 12502- 12510 ,(1987) , 10.1016/S0021-9258(18)45234-9
Vivek Malhotra, Lelio Orci, Benjamin S Glick, Marc R Block, James E Rothman, Role of an N-ethylmaleimide-sensitive transport component in promoting fusion of transport vesicles with cisternae of the Golgi stack Cell. ,vol. 54, pp. 221- 227 ,(1988) , 10.1016/0092-8674(88)90554-5
E. Fries, J. E. Rothman, Transport of vesicular stomatitis virus glycoprotein in a cell-free extract Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 3870- 3874 ,(1980) , 10.1073/PNAS.77.7.3870
Paul Melançon, Benjamin S. Glick, Vivek Malhotra, Peggy J. Weidman, Tito Serafini, Michael L. Gleason, Lelio Orci, James E. Rothman, Involvement of GTP-binding “G” proteins in transport through the Golgi stack Cell. ,vol. 51, pp. 1053- 1062 ,(1987) , 10.1016/0092-8674(87)90591-5
R. Kelly, Pathways of protein secretion in eukaryotes. Science. ,vol. 230, pp. 25- 32 ,(1985) , 10.1126/SCIENCE.2994224
J. Rothman, The golgi apparatus: two organelles in tandem. Science. ,vol. 213, pp. 1212- 1219 ,(1981) , 10.1126/SCIENCE.7268428
G Palade, Intracellular aspects of the process of protein synthesis Science. ,vol. 189, pp. 347- 358 ,(1975) , 10.1126/SCIENCE.1096303
Lelio Orci, Benjamin S. Glick, James E. Rothman, A new type of coated vesicular carrier that appears not to contain clathrin: Its possible role in protein transport within the Golgi stack Cell. ,vol. 46, pp. 171- 184 ,(1986) , 10.1016/0092-8674(86)90734-8
William E. Balch, Benjamin S. Glick, James E. Rothman, Sequential intermediates in the pathway of intercompartmental transport in a cell-free system Cell. ,vol. 39, pp. 525- 536 ,(1984) , 10.1016/0092-8674(84)90459-8