Frequency and characterization of p53 mutations in primary and metastatic human prostate cancer
作者： Winand N. M. Dinjens , Marcel M. Van Der Weiden , Fritz H. Schroeder , Fred T. Bosman , Jan Trapman
关键词: Cancer research 、 Single-strand conformation polymorphism 、 Exon 、 Tumor suppressor gene 、 Primary tumor 、 Prostate 、 Immunohistochemistry 、 Cancer 、 Metastasis 、 Biology
摘要: We have studied the frequency of mutations in p53 gene human prostate cancer. The investigated material consisted 20 primary-tumor tissue specimens, obtained by transurethral resection and specimens 15 lymph-node metastases, at total prostatectomy. applied methods encompassed immunohistochemistry on frozen sections, using monoclonal antibody PAb 1801, single-strand conformation polymorphism (SSCP) analysis, after amplification single exon sequences PCR, exons 5 to 8 gene. mutations, leading aberrantly migrating bands PCR-SSCP were identified direct sequencing PCR product. Immunohistochemical analysis completely confirmative. In primary tumors, found 10% (codons 232 273), metastases 15% 248 273). one case (codon same mutation was both tumor metastasis. Our results show that are infrequent metastatic tumors. addition, they indicate there is no strict correlation between
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