Morin hydrate promotes inner ear neural stem cell survival and differentiation and protects cochlea against neuronal hearing loss.
作者： Qiang He , Zhanwei Jia , Ying Zhang , Xiumin Ren
DOI: 10.1111/JCMM.13005
关键词: Neurite 、 Neurosphere 、 Cellular differentiation 、 Cochlea 、 Inner ear 、 Neural stem cell 、 Chemistry 、 Mechanotransduction 、 Cell biology 、 Ototoxicity 、 Anatomy
摘要: We aimed to investigate the effect of morin hydrate on neural stem cells (NSCs) isolated from mouse inner ear and its potential in protecting neuronal hearing loss. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) bromodeoxyuridine incorporation assays were employed assess viability proliferation vitro NSC culture. The NSCs then differentiated into neurons, which neurosphere formation differentiation evaluated, followed by neurite outgrowth excitability measurements subsequent network. Mechanotransduction cochlea ex vivo culture auditory brainstem responses threshold distortion product optoacoustic emissions amplitude ototoxicity model also measured following gentamicin treatment protective role against Morin improved proliferation, NSCs, promoted network functions. In both models, prevented gentamicin-induced exhibited potent properties promoting growth functional neurons ototoxicity. Our study supports clinical treating
sci-hub.se 参考文章(32)
Kazuo Oshima, Pascal Senn, Stefan Heller, Isolation of sphere-forming stem cells from the mouse inner ear. Methods of Molecular Biology. ,vol. 493, pp. 141- 162 ,(2009) , 10.1007/978-1-59745-523-7_9
Isabella Russo, Sergio Barlati, Francesca Bosetti, Effects of neuroinflammation on the regenerative capacity of brain stem cells. Journal of Neurochemistry. ,vol. 116, pp. 947- 956 ,(2011) , 10.1111/J.1471-4159.2010.07168.X
Zhifeng Yu, Wing Ping Fong, Christopher H. K. Cheng, The Dual Actions of Morin (3,5,7,2',4'-Pentahydroxyflavone) as a Hypouricemic Agent: Uricosuric Effect and Xanthine Oxidase Inhibitory Activity Journal of Pharmacology and Experimental Therapeutics. ,vol. 316, pp. 169- 175 ,(2006) , 10.1124/JPET.105.092684
Andrew P Thomas, Gary St J Bird, György Hajnóczky, Lawrence D Robb‐Gaspers, James W Putney Jr, None, SPATIAL AND TEMPORAL ASPECTS OF CELLULAR CALCIUM SIGNALING The FASEB Journal. ,vol. 10, pp. 1505- 1517 ,(1996) , 10.1096/FASEBJ.10.13.8940296
J. Gálvez, G. Coelho, M. E. Crespo, T. Cruz, M. E. Rodríguez-Cabezas, A. Concha, M. Gonzalez, A. Zarzuelo, Intestinal anti-inflammatory activity of morin on chronic experimental colitis in the rat. Alimentary Pharmacology & Therapeutics. ,vol. 15, pp. 2027- 2039 ,(2001) , 10.1046/J.1365-2036.2001.01133.X
R. Chaddah, M. Arntfield, S. Runciman, L. Clarke, D. van der Kooy, Clonal Neural Stem Cells from Human Embryonic Stem Cell Colonies The Journal of Neuroscience. ,vol. 32, pp. 7771- 7781 ,(2012) , 10.1523/JNEUROSCI.3286-11.2012
Krishnendu Sinha, Pritam Sadhukhan, Sukanya Saha, Pabitra Bikash Pal, Parames C. Sil, Morin protects gastric mucosa from nonsteroidal anti-inflammatory drug, indomethacin induced inflammatory damage and apoptosis by modulating NF-κB pathway. Biochimica et Biophysica Acta. ,vol. 1850, pp. 769- 783 ,(2015) , 10.1016/J.BBAGEN.2015.01.008
Subhashinee S. K. Wijeratne, Mamdouh M. Abou-Zaid, Fereidoon Shahidi, Antioxidant polyphenols in almond and its coproducts Journal of Agricultural and Food Chemistry. ,vol. 54, pp. 312- 318 ,(2006) , 10.1021/JF051692J
Zhaobing Qin, Melissa Wood, John J. Rosowski, Measurement of Conductive Hearing Loss in Mice Hearing Research. ,vol. 263, pp. 93- 103 ,(2010) , 10.1016/J.HEARES.2009.10.002
Bifeng Pan, Gwenaelle S. Géléoc, Yukako Asai, Geoffrey C. Horwitz, Kiyoto Kurima, Kotaro Ishikawa, Yoshiyuki Kawashima, Andrew J. Griffith, Jeffrey R. Holt, TMC1 and TMC2 are components of the mechanotransduction channel in hair cells of the mammalian inner ear. Neuron. ,vol. 79, pp. 504- 515 ,(2013) , 10.1016/J.NEURON.2013.06.019