Colocalization of TrkB and brain-derived neurotrophic factor proteins in green-red-sensitive cone outer segments.
作者： Adriana Di Polo , Garth M. Bray , Albert J. Aguayo , Li Cheng
DOI:
关键词: Trk receptor 、 Cell biology 、 Tropomyosin receptor kinase B 、 Neurotrophin 、 Low-affinity nerve growth factor receptor 、 Tropomyosin receptor kinase C 、 Endocrinology 、 Internal medicine 、 Tropomyosin receptor kinase A 、 Brain-derived neurotrophic factor 、 Biology 、 Neurotrophic factors
摘要: PURPOSE. To examine the distribution of neurotrophins (NTs) and their catalytic receptors in adult rat photoreceptors. METHODS. Immunocytochemistry Western blot analyses were performed using primary antibodies raised against NTs (nerve growth factor [NGF], brain-derived neurotrophic [BDNF], NT-3, NT-4/5) NT (TrkA, TrkB, TrkC, p75NTR). Double-labeling retinal sections with opsin-specific was to identify each photoreceptor type. Competitive experiments excess recombinant or Trk confirmed binding specificity antibody. RESULTS. TrkB BDNF immunoreactivity colocalized cone outer segments. detected all green-red‐sensitive cones, but not blue-UV cones rods, other any types. CONCLUSIONS. The findings suggest a specific role for through its signaling receptor function maintenance green-red predominant type retina. (Invest Ophthalmol Vis Sci. 2000;41:4014 ‐ 4021)
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