Methods and kits for performing nucleic acid hybridization assays
作者： Yitzhak Stabinsky
DOI:
关键词: Sequence (biology) 、 Molecular biology 、 Biochemistry 、 Nucleic acid thermodynamics 、 Biology 、 Nucleic acid sequence 、 Polynucleotide
摘要: A method for the isolation and quantitative detection of a selected single-stranded target polynucleotide from solution. The is hybridized in solution to mediator polynucleotide, probe an immobilized sequence. sequence comprises first complementary portion second nucleotide which carries detectable label, necleotide target. by attachment solid support, and, through hybridization functions immobilize entire polynucleotide/target polynucleotide/probe "sandwich".
google.com.tr参考文章(8)
Alan David Blair Malcolm, Jean Lesley Nicolas, Method of detecting a polynucleotide sequence and labelled polynucleotides useful therein ,(1984)
W. Robert Frederick Md Us Blakesley, A. John Myersville Md Us Thompson, Immobilization of nucleic acids ,(1984)
Stanley Falkow, Stephen L. Moseley, Specific DNA probes in diagnostic microbiology ,(1980)
Hans E. Soderlund, Tuula M. Ranki, Detection of microbial nucleic acids by a one-step sandwich hybridization test ,(1982)
Serge L. Beaucage, Marvin H. Caruthers, Phosphoramidite compounds and processes ,(1981)
David Andrew Shafritz, Diagnostic test for hepatitis B virus ,(1981)
David John Chiswell, Detecting polynucleotide sequences ,(1985)
Donald M. Crothers, Nanibhushan Dattagupta, Peter M. M. Rae, Testing dna samples for particular nucleotide sequences ,(1984)