Oligonucleotide library for detecting rna transcripts and splice variants that populate a transcriptome

作者: Simchon Faigler , Alon Wasserman , Eli Mintz , Liat Mintz , Avi Shoshan

DOI:

关键词: GeneTranscriptomeRNAGeneticsOligonucleotideDNA-encoded chemical libraryGenomeGene expression profilingTranscription (biology)Biology

摘要: The present invention provides oligonucleotide libraries capable of detecting RNA transcripts and splice variants which populate a transcriptome are transcribed from genes or transcription units that the corresponding genome. also arrays generated methods using in various detection systems expression profiling studies. Antisense molecules double-stranded interfering RNAs, types oligonucleotides, based on oligonucleotides disclosed herein provided.

参考文章(11)
Larry Croft, Soeren Schandorff, Francis Clark, Kevin Burrage, Peter Arctander, John S Mattick, None, ISIS, the intron information system, reveals the high frequency of alternative splicing in the human genome Nature Genetics. ,vol. 24, pp. 340- 341 ,(2000) , 10.1038/74153
Mor Amitai, Avner Magen, Raveh Avraham Gill-More, Eran Halperin, Sarah Rachel Pollock, Method and apparatus for mRNA assembly ,(1998)
M. Schena, D. Shalon, R. W. Davis, P. O. Brown, Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray Science. ,vol. 270, pp. 467- 470 ,(1995) , 10.1126/SCIENCE.270.5235.467
Lia V Varga, Sára Tóth, István Novák, András Falus, Antisense strategies: functions and applications in immunology Immunology Letters. ,vol. 69, pp. 217- 224 ,(1999) , 10.1016/S0165-2478(99)00082-6
David J. Lockhart, Helin Dong, Michael C. Byrne, Maximillian T. Follettie, Michael V. Gallo, Mark S. Chee, Michael Mittmann, Chunwei Wang, Michiko Kobayashi, Heidi Norton, Eugene L. Brown, Expression monitoring by hybridization to high density oligonucleotide arrays Nature Biotechnology. ,vol. 14, pp. 1675- 1680 ,(1996) , 10.1038/NBT1296-1675
Lin Zhang, Wei Zhou, Victor E Velculescu, Scott E Kern, Ralph H Hruban, Stanley R Hamilton, Bert Vogelstein, Kenneth W Kinzler, Gene expression profiles in normal and cancer cells Science. ,vol. 276, pp. 1268- 1272 ,(1998) , 10.1126/SCIENCE.276.5316.1268
Sayda M Elbashir, Winfried Lendeckel, Thomas Tuschl, RNA interference is mediated by 21- and 22-nucleotide RNAs Genes & Development. ,vol. 15, pp. 188- 200 ,(2001) , 10.1101/GAD.862301
Phillip D Zamore, Thomas Tuschl, Phillip A Sharp, David P Bartel, RNAi: Double-Stranded RNA Directs the ATP-Dependent Cleavage of mRNA at 21 to 23 Nucleotide Intervals Cell. ,vol. 101, pp. 25- 33 ,(2000) , 10.1016/S0092-8674(00)80620-0
Daniel Gautheret, Olivier Poirot, Fabrice Lopez, Stéphane Audic, Jean-Michel Claverie, Alternate Polyadenylation in Human mRNAs: A Large-Scale Analysis by EST Clustering Genome Research. ,vol. 8, pp. 524- 530 ,(1998) , 10.1101/GR.8.5.524