Spectroscopic studies of D-α-tocopherol concentration-induced transformation in egg phosphatidylcholne vesicles
作者： Krzysztof Dwiecki , Paweł Górnas , Agnieszka Wilk , Małgorzata Nogala-Kałucka , Krzysztof Polewski
DOI: 10.2478/S11658-006-0059-6
关键词: Fluorescence 、 Chemistry 、 Membrane 、 Quenching (fluorescence) 、 Micelle 、 Vesicle 、 Biophysics 、 Dynamic light scattering 、 Phosphatidylcholine 、 Biochemistry 、 Tocopherol
摘要: The effects of embedding up to 60 mol% α-tocopherol (α-Toc) on the morphology and structure egg phosphatidylcholine (PC) membrane were studied using spectroscopic techniques. resulting vesicles subjected turbidometric dynamic light scattering measurements evaluate their size distribution. α-Toc intrinsic fluorescence its quenching was used estimate tocopherol position in membrane. Optical microscopy visualize morphological changes during inclusion into 2 mg/ml PC incorporation 15 molecules is accompanied by a linear increase intensity simultaneous formation larger, multilamellar vesicles. Increasing concentration above 20 induced structural leading disappearance micrometer-sized small unilamellar ranging from 30 120 nm, mixed micelles non-lamellar structures.
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sci-hub.se 参考文章(62)
Valerian E. Kagan, Rumyana A. Bakalova, Elena E. Serbinova, Tsanko S. Stoytchev, Fluorescence measurements of incorporation and hydrolysis of tocopherol and tocopheryl esters in biomembranes. Methods in Enzymology. ,vol. 186, pp. 355- 367 ,(1990) , 10.1016/0076-6879(90)86129-J
Ernö Pretsch, Philippe Bühlmann, Martin Badertscher, 13C NMR Spectroscopy Springer Berlin Heidelberg. pp. 1- 88 ,(2009) , 10.1007/978-3-540-93810-1_4
Valerian E. KAGAN, Peter J. QUINN, The interaction of α-tocopherol and homologues with shorter hydrocarbon chains with phospholipid bilayer dispersions FEBS Journal. ,vol. 171, pp. 661- 667 ,(1988) , 10.1111/J.1432-1033.1988.TB13837.X
Gabriela Gramlich, Jiayun Zhang, Werner M. Nau, Diffusion of α-tocopherol in membrane models: Probing the kinetics of vitamin E antioxidant action by fluorescence in real time Journal of the American Chemical Society. ,vol. 126, pp. 5482- 5492 ,(2004) , 10.1021/JA039845B
Thomas Kaasgaard, Ole G. Mouritsen, Kent Jørgensen, Lipid domain formation and ligand–receptor distribution in lipid bilayer membranes investigated by atomic force microscopy FEBS Letters. ,vol. 515, pp. 29- 34 ,(2002) , 10.1016/S0014-5793(02)02391-8
Alicia Grau, Antonio Ortiz, Dissimilar protection of tocopherol isomers against membrane hydrolysis by phospholipase A2. Chemistry and Physics of Lipids. ,vol. 91, pp. 109- 118 ,(1998) , 10.1016/S0009-3084(97)00101-1
H.-W Chen, M.-T Chiang, C.-Y Wang, C.-K Lii, Inhibition of tert-butyl hydroperoxide-induced cell membrane bleb formation by α-tocopherol and glutathione Food and Chemical Toxicology. ,vol. 38, pp. 1089- 1096 ,(2000) , 10.1016/S0278-6915(00)00097-1
J. Salgado, J. Villalain, J.C. Gomezfernandez, α-Tocopherol Interacts with Natural Micelle-Forming Single-Chain Phospholipids Stabilizing the Bilayer Phase Archives of Biochemistry and Biophysics. ,vol. 306, pp. 368- 376 ,(1993) , 10.1006/ABBI.1993.1525
Fabienne Nacka, Maud Cansell, Philippe Méléard, Nicole Combe, Incorporation of α-tocopherol in marine lipid-based liposomes: in vitro and in vivo studies Lipids. ,vol. 36, pp. 1313- 1320 ,(2001) , 10.1007/S11745-001-0846-X
Robert T. LaLonde, Shan Xie, Glutathione and N-acetylcysteine inactivations of mutagenic 2(5H)-furanones from the chlorination of humics in water. Chemical Research in Toxicology. ,vol. 6, pp. 445- 451 ,(1993) , 10.1021/TX00034A010