Insertional Mutations in Transgenic Mice

摘要: Publisher Summary In contrast to the rapid rate of progress in developmental genetics invertebrate species such as Drosophila melanogaster or nematode C. elegans , genetic analysis mammalian development remains a difficult undertaking. Several approaches are now making it possible identify, and analyze at molecular level, genes that potentially important development. This chapter presents mutations caused by insertion foreign DNA viruses into mouse germ line. It describes methods identify insertional causing recessive prenatal lethality, summarizes their phenotypic effects. The presence lethal mutation transgenic line can be discerned failure obtain homozygous animals among progeny an intercross. cases which host sequences flanking transgene have already been cloned, probe may used homozygotes, based on Southern blot analysis. other cases, identification mice has usually relied quantitation copy number: carry twice number copies do heterozygous mice. relative estimated either dot hybridization, each case comparing signal obtained with for endogenous gene.