[31] Very long chain fatty acid α-hydroxylase from brain
作者： Yasuo Kishimoto
DOI: 10.1016/S0076-6879(78)52033-8
关键词: Organic chemistry 、 Chelation 、 Substrate (chemistry) 、 Fatty acid 、 Chemistry 、 Very long chain fatty acid 、 Rat brain 、 Tritium 、 Copper 、 Chromatography 、 Lignoceric acid
摘要: Publisher Summary This chapter focuses on the fatty acid α-hydroxylase. The rat brain α-hydroxylase shows a pH optimum of 7.8. mentions two different procedures that are routinely used. One is based selective formation insoluble copper chelate hydroxy acids and other release radioactivity to water from specifically labeled its 2R-position with tritium. method reliable, specific, reproducible; however, it very time-consuming tedious requires well trained personnel run. On hand, second method, tritium substrate, relatively simple quick. However, not as reliable method: in some instances, [2R- 3 H] lignoceric released soluble compound even when no a-hydroxylation activity was detected by method. enzymic appears be severely inhibited any trace detergent.
sci-hub.se 参考文章(11)
S Murad, Y Kishimoto, Alpha hydroxylation of lignoceric acid to cerebronic acid during brain development. Diminished hydroxylase activity in myelin-deficient mouse mutants. Journal of Biological Chemistry. ,vol. 250, pp. 5841- 5846 ,(1975) , 10.1016/S0021-9258(19)41129-0
S Murad, R H Chen, Y Kishimoto, alpha-Hydroxylation of fatty acids in brain. Substrate specificity and deuterium isotope effect. Journal of Biological Chemistry. ,vol. 252, pp. 5206- 5210 ,(1977) , 10.1016/S0021-9258(19)63332-6
G D Strycharz, Y Kishimoto, S Murad, Alpha-Hydroxylation of lignoceric and nervonic acids in the brain. Effects of altered thyroid function on postnatal development of the hydroxylase activity. Journal of Biological Chemistry. ,vol. 251, pp. 5237- 5241 ,(1976) , 10.1016/S0021-9258(17)33152-6
S-C Tsai, J Avigan, D Steinberg, Studies on the α Oxidation of Phytanic Acid by Rat Liver Mitochondria Journal of Biological Chemistry. ,vol. 244, pp. 2682- 2692 ,(1969) , 10.1016/S0021-9258(18)83452-4
Kiyoshi Tatsumi, Yasuo Kishimoto, Charles Hignite, Stereochemical aspects of synthetic and naturally occurring 2-hydroxy fatty acids Archives of Biochemistry and Biophysics. ,vol. 165, pp. 656- 664 ,(1974) , 10.1016/0003-9861(74)90294-X
Motonori Hoshi, Yasuo Kishimoto, Synthesis of Cerebronic Acid from Lignoceric Acid by Rat Brain Preparation Journal of Biological Chemistry. ,vol. 248, pp. 4123- 4130 ,(1973) , 10.1016/S0021-9258(19)43848-9
Ramesh C. Arora, Yuh-Nan Lin, Norman S. Radin, The inhibitor-sensitive sites of galactosyl ceramide galactosidase Archives of Biochemistry and Biophysics. ,vol. 156, pp. 77- 83 ,(1973) , 10.1016/0003-9861(73)90343-3
Kiyoshi Tatsumi, Saood Murad, Yasuo Kishimoto, Mechanism and stereospecificity of α-hydroxylation of lignoceric acid in rat brain Archives of Biochemistry and Biophysics. ,vol. 171, pp. 87- 92 ,(1975) , 10.1016/0003-9861(75)90010-7
Pierre Morell, Elvira Costantino-Ceccarini, Norman S. Radin, The biosynthesis by brain microsomes of cerebrosides containing nonhydroxy fatty acids Archives of Biochemistry and Biophysics. ,vol. 141, pp. 738- 748 ,(1970) , 10.1016/0003-9861(70)90192-X
Saood Murad, Kishimoto Yasuo, ga-Hydroxylation of fatty acid in brain effects of cerebroside components on the synthesis of cerebronic acid Biochimica et Biophysica Acta. ,vol. 488, pp. 102- 111 ,(1977) , 10.1016/0005-2760(77)90127-8