Further characterization of proteins assembled by vesicular stomatitis virus from human tumor cells.

摘要: Vesicular stomatitis virus (VSV), when reproduced in human tumor cell lines, assembled a specific subset of cell-derived proteins. These were detected by 35S]methionine labeling cells prior to infection and subsequent immunoprecipitation VSV grown these cells, as well direct labeled extracts with antiserum directed against the VSV-assembled Their molecular weight (Mr) ranged between 15K 180K; larger proteins glycosylated. Two major protein species (gp88 gp130) common all four lines used (HeLa-cervical carcinoma, T47D-breast HMB2 SK1477-two melanoma lines). Proteins other weights only one or two lines. The (even absence VSV) shed large particulate material which had contained same spectrum that VSV. component an Mr 30K. Some might possibly serve markers. It is also conceivable be products defective endogenous retroviruses.