Vesicular stomatitis virus produced from infected LSTRA lymphoma cells bear tyrosine protein kinase activity (p56).
作者： S Fischer , A Flamand , R Fagard , J F Chich , J P Piau
DOI: 10.1016/S0021-9258(17)38579-4
关键词: Viral membrane 、 Rhabdoviridae 、 Vesicular stomatitis virus 、 Tyrosine 、 Biology 、 Protein kinase A 、 Phosphorylation 、 Tyrosine kinase 、 Biochemistry 、 Vesicular Stomatitis
摘要: Murine LSTRA lymphoma cells contain a very active tyrosine protein kinase of 56 kDa (p56) which is not related to any the other known kinases. In past purification and characterization p56 have been hampered because low amount this in membranes. study, we utilized different approach for consisted trapping membrane vesicular stomatitis virus. Incubation virions with [gamma-32P]ATP resulted phosphorylation on residues. Moreover, phosphopeptide digest profile virus-p56 was identical that observed authentic LSTRA-p56. The from such could be resolved proteins by two-dimensional gels, furthermore, used prepare several antisera directed against p56.
sci-hub.st 参考文章(18)
G. Gacon, S. Gisselbrecht, J.P. Piau, J.P. Boissel, J. Tolle, S. Fischer, High level of tyrosine protein kinase in a murine lymphoma cell line induced by Moloney leukemia virus. The EMBO Journal. ,vol. 1, pp. 1579- 1582 ,(1982) , 10.1002/J.1460-2075.1982.TB01358.X
J E Casnellie, M L Harrison, K E Hellstrom, E G Krebs, A lymphoma cell line expressing elevated levels of tyrosine protein kinase activity. Journal of Biological Chemistry. ,vol. 258, pp. 10738- 10742 ,(1983) , 10.1016/S0021-9258(17)44518-2
G M Clinton, N G Guerina, H Y Guo, A S Huang, Host-dependent phosphorylation and kinase activity associated with vesicular stomatitis virus. Journal of Biological Chemistry. ,vol. 257, pp. 3313- 3319 ,(1982) , 10.1016/S0021-9258(19)81111-0
PH O'Farrell, High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry. ,vol. 250, pp. 4007- 4021 ,(1975) , 10.1016/S0021-9258(19)41496-8
A F Voronova, J E Buss, T Patschinsky, T Hunter, B M Sefton, Characterization of the protein apparently responsible for the elevated tyrosine protein kinase activity in LSTRA cells. Molecular and Cellular Biology. ,vol. 4, pp. 2705- 2713 ,(1984) , 10.1128/MCB.4.12.2705
F. Bussereau, A. Flamand, D. Pese-Part, Reproducible plaquing system for rabies virus in CER cells. Journal of Virological Methods. ,vol. 4, pp. 277- 282 ,(1982) , 10.1016/0166-0934(82)90074-X
Jan Závada, Alice S. Huang, Further characterization of proteins assembled by vesicular stomatitis virus from human tumor cells. Virology. ,vol. 138, pp. 16- 25 ,(1984) , 10.1016/0042-6822(84)90143-0
Amiya K. Banerjee, Sue A. Moyer, Dennis P. Rhodes, Studies on the in vitro adenylation of RNA by vesicular stomatitis virus Virology. ,vol. 61, pp. 547- 558 ,(1974) , 10.1016/0042-6822(74)90289-X
G. Gacon, R. Fagard, J.P. Boissel, S. Fischer, L. Reibel, J.P. Piau, G. Schapira, P.M. Comoglio, Identification of a 58,000 Daltons phosphoprotein with tyrosine protein kinase activity in a murine lymphoma cell line Biochemical and Biophysical Research Communications. ,vol. 122, pp. 563- 570 ,(1984) , 10.1016/S0006-291X(84)80070-4
J. E. Casnellie, L. E. Gentry, L. R. Rohrschneider, E. G. Krebs, Identification of the tyrosine protein kinase from LSTRA cells by use of site-specific antibodies. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 81, pp. 6676- 6680 ,(1984) , 10.1073/PNAS.81.21.6676