Studies on the in vitro adenylation of RNA by vesicular stomatitis virus

摘要: Abstract Polyadenylate sequences present in RNA synthesized vitro by purified vesicular stomatitis virus (VSV) are located at the 3′-termini of chains. These heterogeneous length, averaging 140 bases, and composed entirely AMP. VSV from different cell lines synthesizes containing poly(A) complete removal viral envelope proteins G M does not affect its synthesis. No synthesis was observed with ATP as only substrate either presence or absence exogenous primers. Cordycepin triphosphate did inhibit substitute for reaction. Poly(A) added to product RNAs during after their release genome template. A possible mechanism adenylation process is discussed.