A lymphoma cell line expressing elevated levels of tyrosine protein kinase activity.

摘要: The major in vitro substrate for a tyrosine protein kinase the particular fraction of lymphoma cell line LSTRA is approximately 58,000 daltons (pp58). In order to determine if this phosphorylation unique cells, particulate fractions from normal mouse T lymphocytes and another line, YAC-1, were examined presence pp58 phosphorylation. These two types found contain phosphorylation, although elevated 20-40-fold cells over that or YAC-1 cells. A comparison was also made levels activity among these three types. Tyrosine measured using an exogenously added synthetic peptide vivo by determining cellular phosphotyrosine levels. results determinations indicate contains level activity.