A tyrosine kinase related to pp60c-src is associated with membranes of Electrophorus electricus electric organ.
作者： C G Shores , M E Cox , P F Maness
DOI: 10.1016/S0021-9258(18)47958-6
关键词:
摘要: Abstract A tyrosine-specific protein kinase immunologically related to pp60c-src, the cellular homolog of Rous sarcoma virus-transforming protein, was expressed at elevated levels in electric organ eel Electrophorus electricus. The phosphorylated antibodies reactive with pp60c-src tyrosine residues immune complex assays and associated membranes enriched acetylcholine receptors. recognized by anti-pp60c-src endogenous membrane phosphorylation reactions shown have a relative molecular mass 57 kDa two-dimensional gel electrophoresis. In 57-kDa threonine distinct from organ. purified 844-fold chromatography on omega-aminohexyl agarose, phosphocellulose, casein-Sepharose. Threonine activity immunoprecipitates not observed fractions after first step. Incubation casein Sepharose fraction [gamma-32P]ATP-Mn2+ solution resulted only protein. Phosphorylation occurred solely tyrosine, suggesting that is capable autophosphorylation. structural functional properties indicate member src subfamily kinases closely pp60c-src.
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