A lymphoma protein with an in vitro site of tyrosine phosphorylation homologous to that in pp60src.
作者： J E Casnellie , M L Harrison , K E Hellstrom , E G Krebs
DOI: 10.1016/S0021-9258(19)45309-X
关键词: Tyrosine 、 Protease 、 Tyrosine phosphorylation 、 Protein kinase A 、 Biochemistry 、 Biology 、 In vitro 、 Rous sarcoma virus 、 Peptide sequence 、 Molecular biology 、 Proteolysis 、 Cell biology
摘要: The major in vitro substrate for a tyrosine protein kinase the particulate fraction of lymphoma cell line LSTRA is molecular weight 58,000 (pp58) (Casnellie, J. E. Harrison, M. L., Pike, L. J., Hellstrom, K. E., and Krebs, G. (1982) Proc. Natl. Acad. Sci. U. S. A. 79, 282-286). In order to determine if this was related pp60src, transformation-specific from Rous sarcoma virus, partial proteolysis maps 32P-labeled pp58 pp60src were prepared using Staphylococcus aureus V8 protease papain. clearly different, indicating distinct pp60src. However characterization tryptic fragment containing single site phosphorylation has revealed that amino acid sequence around extremely homologous to, not identical with
elsevier.com
sci-hub.st 参考文章(12)
K Beemon, T Hunter, Characterization of Rous sarcoma virus src gene products synthesized in vitro. Journal of Virology. ,vol. 28, pp. 551- 566 ,(1978) , 10.1128/JVI.28.2.551-566.1978
M S Collett, E Erikson, R L Erikson, Structural analysis of the avian sarcoma virus transforming protein: sites of phosphorylation. Journal of Virology. ,vol. 29, pp. 770- 781 ,(1979) , 10.1128/JVI.29.2.770-781.1979
J. E. Smart, H. Oppermann, A. P. Czernilofsky, A. F. Purchio, R. L. Erikson, J. M. Bishop, Characterization of sites for tyrosine phosphorylation in the transforming protein of Rous sarcoma virus (pp60v-src) and its normal cellular homologue (pp60c-src) Proceedings of the National Academy of Sciences of the United States of America. ,vol. 78, pp. 6013- 6017 ,(1981) , 10.1073/PNAS.78.10.6013
N. Kitamura, A. Kitamura, K. Toyoshima, Y. Hirayama, M. Yoshida, Avian sarcoma virus Y73 genome sequence and structural similarity of its transforming gene product to that of Rous sarcoma virus Nature. ,vol. 297, pp. 205- 208 ,(1982) , 10.1038/297205A0
James C. Neil, Jacques Ghysdael, Peter K. Vogt, John E. Smart, Homologous tyrosine phosphorylation sites in transformation-specific gene products of distinct avian sarcoma viruses Nature. ,vol. 291, pp. 675- 677 ,(1981) , 10.1038/291675A0
L. R. Rohrschneider, R. N. Eisenman, C. R. Leitch, Identification of a Rous sarcoma virus transformation-related protein in normal avian and mammalian cells. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 76, pp. 4479- 4483 ,(1979) , 10.1073/PNAS.76.9.4479
H. Oppermann, A. D. Levinson, H. E. Varmus, L. Levintow, J. M. Bishop, Uninfected vertebrate cells contain a protein that is closely related to the product of the avian sarcoma virus transforming gene (src). Proceedings of the National Academy of Sciences of the United States of America. ,vol. 76, pp. 1804- 1808 ,(1979) , 10.1073/PNAS.76.4.1804
M. S. Collett, E. Erikson, A. F. Purchio, J. S. Brugge, R. L. Erikson, A normal cell protein similar in structure and function to the avian sarcoma virus transforming gene product. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 76, pp. 3159- 3163 ,(1979) , 10.1073/PNAS.76.7.3159
David J. Shealy, Raymond L. Erikson, Human cells contain two forms of pp60c-src. Nature. ,vol. 293, pp. 666- 669 ,(1981) , 10.1038/293666A0
J. E. Casnellie, M. L. Harrison, L. J. Pike, K. E. Hellstrom, E. G. Krebs, Phosphorylation of synthetic peptides by a tyrosine protein kinase from the particulate fraction of a lymphoma cell line Proceedings of the National Academy of Sciences of the United States of America. ,vol. 79, pp. 282- 286 ,(1982) , 10.1073/PNAS.79.2.282