Sites of in vivo phosphorylation of the T cell tyrosine protein kinase in LSTRA cells and their alteration by tumor-promoting phorbol esters.
作者： J E Casnellie
DOI: 10.1016/S0021-9258(18)48012-X
关键词:
摘要: Abstract The LSTRA cell line contains an elevated level of a tyrosine protein kinase apparent molecular weight 56,000 (pp56Tcell). Analysis the tryptic fragments this labeled in vivo with 32P shows that it four sites phosphorylation and one site serine phosphorylation. Two are also vitro when membranes incubated [gamma-32P]ATP. One is (site 1) identical sequence major transforming Rous sarcoma virus. pp56Tcell from cells treated 4 beta-phorbol 12 beta-myristate 13 alpha-acetate (PMA) reveals agent induces at least new Treatment PMA causes selective reduction 1. Thus reduces amount phosphate
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