Identification of the tyrosine protein kinase from LSTRA cells by use of site-specific antibodies.

摘要: Abstract The lymphoma cell line LSTRA contains an elevated level of tyrosine protein kinase activity. It has been suggested that this activity is due to the presence a phosphoprotein with molecular weight 56,000 (pp56, formerly referred as 58,000-dalton protein). This paper describes preparation antibodies against pp56 through use synthetic peptide sequence around site phosphorylation in pp56, which identical pp60src. These antipeptide specifically immunoprecipitated 32P-labeled from detergent extracts cells. In immunoblotting experiments, was major antigen detected particulate fraction cells by antibodies. The were also used show greatly Incubation extract resulted inhibition most results indicate whose enzyme may be member large family kinases involved regulation growth.