Purification of the LSTRA tyrosine protein kinase (p56lck)
作者： Huynh TAN , Siegmund FISCHER , Remi FAGARD
DOI: 10.1111/J.1432-1033.1988.TB13856.X
关键词: Tyrosine 、 Polyacrylamide gel electrophoresis 、 Antibody 、 Murine leukemia virus 、 Enzyme 、 Biology 、 Isoelectric focusing 、 Protein kinase A 、 Cell culture 、 Biochemistry
摘要: p56lck, a p60src related tyrosine protein kinase was recently described in murine lymphoma cell line (LSTRA), several human lymphomas and normal peripheral lymphocytes. We have purifed p56lck to homogeneity by electrofocusing followed two-step SDS/polyacrylamide gel electrophoresis. The pure identical the p56 characterized LSTRA cells used raise antibodies rabbits. This simple procedure is applicable for rapid purification of minor proteins.
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