Human cervical carcinoma cell lines contain an antigen identical to the tumor-specific 75 kDa antigen of HeLa cells: detection by viral acquisition.
作者： Steve S.-L. Chen , Alice S. Huang
DOI: 10.1016/0166-0934(89)90018-9
关键词: Biology 、 HeLa 、 Vesicular stomatitis virus 、 Molecular biology 、 Antigen 、 Carcinoma 、 Virology 、 Rhabdoviridae 、 Papillomaviridae 、 Cell culture 、 Virus
摘要: Purified vesicular stomatitis virus grown in the human cervical carcinoma HeLa cell line, VSV(HeLa), contains a 75 kDa tumor-specific antigen, detectable by immunoblotting of electrophoretically separated proteins with rabbit antiserum made against whole cells. Nearly identical results were obtained VSV tumorigenic hybrid ESH-5L cells, but not matched non-tumorigenic ESH-5E Growth 4 other independently isolated lines led to concentration same antigen VSV. Infection 2 did lead detection this antigen. The expression correlated directly amount RNA from papillomavirus integrated DNA these irrespective whether was type 16 or 18.
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