Sperm Autoantigens and Fertilization. I. Eftects of Antisperm Autoantibodies on Rouleaux Formation, Viability, and Acrosome Reaction of Guinea Pig Spermatozoa

摘要: Fab and IgG were isolated from the sera of 1) guinea pigs immunized with their own epididymal spermatozoa or testis homogenate in complete Freund’s adjuvant, 2) normal pigs. Their effects on rouleaux formation, viability, acrosomal reaction pig studied vitro. Monovalent (Fab) antibodies to homogenate, but not serum, rapidly dispersed sperm a dosedependent time-dependent manner. The effect was reversed by addition prevented if antibody previously absorbed spleen cells Mycobacterium tuberculosis. Compared less viable, rate significantly reduced. Contrary expectation, bivalent (IgG) did agglutinate rouleaux. It head-to-head agglutination prolonged viability. Most significantly, acrosome completely inhibited presence 3.2 X 10’ molecules (to surface antigens spermatozoa) per spermatozoon. results strongly implicate testicular cell autoantigens adhesion phenomenon leading induction capacitation and/or reaction. We further suggest that, pig; prevent premature