Selective stimulation of the synthesis of an 80,000-dalton protein by calcium ionophores.
作者： F.S. Wu , Y.C. Park , D. Roufa , A. Martonosi
DOI: 10.1016/S0021-9258(19)69197-0
关键词: Molecular biology 、 Ionomycin 、 Ionophore 、 HeLa 、 Methionine 、 Biology 、 Cytoplasm 、 Messenger RNA 、 Calcium Ionophores 、 RNA
摘要: Brief exposure of cultured chicken pectoralis muscle cells to ionomycin or A23187 selectively increases the rate incorporation [35S]methionine into an 80,000-dalton protein was also observed upon cell-free translation poly(A)-enriched RNA isolated from ionomycin-treated, as compared with control, cultures. These observations suggest that cellular concentration mRNA, which codes for protein. The effect is probably mediated through increase in cytoplasmic [Ca2+] caused by ionophore. A similar fibroblasts, HeLa cells, mouse LSP and monkey kidney CV1 cells.
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